PCR Refresher Teacher CLPL Workshop
The University of Edinburgh , Edinburgh EH25 9RG , United Kingdom
Every participating teacher will be given a goodie bag containing a printed copy of our DNA Gel Electrophoresis Wall Tank for use in the classroom.
Do you want to...
- Improve confidence in performing and teaching PCR and gel electrophoresis?
- Build on skills of micropipetting and agarose gel electrophoresis?
- Get some ideas on how you can teach PCR and gel electrophoresis the classroom?
Join the Easter Bush Science Outreach Centre (EBSOC) team to get a unique chance to do our PCR Masterclass: A Question of Taste. You will update your knowledge about the science behind these practical techniques, tour the Roslin Institute as well as getting some hands-on practice.
Every participating teacher will be given a goodie bag containing a gel-electrophoresis activity for use in the classroom.
Summary of workshop
Using your own DNA, you will discover and compare your version of a bitter taste receptor gene (genotype) to your ability to taste a bitter compound called phenylthiocarbamide (PTC) (phenotype). You will use cutting-edge equipment to develop expertise and carry out the modern laboratory techniques of DNA extraction, PCR, restriction enzyme digestion and DNA gel electrophoresis. You will update and deepen your knowledge about these practical techniques, tour the Roslin Institute and collaborate with colleagues to reflect on your own practice.
- DNA extraction
- Restriction digest
- DNA gel electrophoresis
- DNA extraction of your own cheek cells using centrifugation to pellet your cells
- Hands-on PCR activity and using micro-pipetting to set up PCR
- Restriction digest to determine your genotype
- Electrophoresis on agarose gels
- Analysis and interpretation of results
Free parking is available at Easter Bush Campus in The Roslin Institute and Veterinary Teaching Building car parks. Please provide your car registration at Charnock Bradley Building reception when you check in for the event.
The campus is well-served by Lothian buses (buses 15, some 37 services, the X47 (but not the 47) and the 67) – please see www.lothianbuses.com. For more information please download our How to Get Here document
Accessibility and Inclusion
We are keen to make our events as inclusive as possible - please email us at firstname.lastname@example.org or call the Easter Bush Science Outreach Centre team on 0131 651 9679 before booking if you would like to find out more about accessibility or usability.
Easter Bush Science Outreach Centre
Charnock Bradley Building (next to The Roslin Institute)
Easter Bush Campus
The University of Edinburgh, Midlothian